National Science Foundation - USA - Chemical Measurement & Imaging

Grant Agreement number: 1508240
Period covered - start date: 01/09/2015
Period covered - end date: 31/08/2018
Project's coordinators: Carol Hirschmugl and Sara Patch, University of Wisconsin-Milwaukee
NMBU principal researcher: Achim Kohler 

 

The overall objective of this proposal is to implement Fourier transform infrared (FTIR) spectro-micro-tomography (SmT) for samples that are extended in two dimensions and to implement limited angle reconstruction algorithms and optimize spectrochemical analysis methods to enhance spectral and image quality. The rationale for this proposal is that data from improved novel chemical imaging techniques combined with advanced data analysis may be able to provide new views of of intact, (bio)chemically complex systems. This project focuses on three-dimensional imaging of biologically relevant samples (e.g, tissues, extended cell cultures, biofilms) or to materials for bioenergy (e.g, algae, wood). FTIR-SmT aims to generate high contrast results through the acquisition of rich IR absorption spectra for organic materials. Such a capability for sensing chemical distributions for intact samples may provide the foundation for fundamentally new approaches in bioanalytical chemistry, well beyond these studies.

 

Literature:

Schofield A.J., Blümel R., Kohler A., Lukacs R., Hirschmugl C.J.
Extracting pure absorbance spectra in infrared microspectroscopy by modeling absorption bands as Fano resonances.
The Journal of Chemical Physics 150 (2019) 154124.

Azarfar G., Aboualizadeh E., Walter N.M., Ratti S., Olivieri C., Norici A., Nasse M., Kohler A., Giordano M., Hirschmugl C.J.
Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging.
Analyst 143 (2018) 4674.