Synchrotron pollen

SOLEIL, French national synchrotron facility

Project number: 20120345
Period covered - start date: 01.07.2012
Period covered - end date: 31.12.2012
Project's coordinator: NMBU and SOLEIL

Collaboration:
SOLEIL synchrotron, SMIS beamline
Date of experiments: 04.-09.12.2012.

FTIR microspectroscopy of samples, that are of the same order of size as the wavelength, such as microorganisms and cells, is impaired by strong scattering phenomena. The scattering effects have been considered as a major obstacle for the reliable interpretation and further use of the IR spectra in biological and biomedical science. We have recently started to develop a 3-dimensional scatter codes for estimating scatter contributions for the scattering at arbitrarily shaped cells and energy conversion materials. However, experimental measurement of scattering properties of cells and tissues is required to develop and validate these models. Due to their valuable properties (low water content and shapepersistent morphology) pollen grains are an ideal real-world biological model system for characterization of scattering phenomena. Therefore, high-quality FTIR spectra of pollen grains will provide important experimental data for modelling of strongly scattering biological samples.

Literature:

Brandsrud M.A., Blümel R., Heitmann Solheim J., Kohler A.
The effect of deformation of absorbing scatterers on Mie-type signatures in infrared microspectroscopy
Scientific Reports 11 (2021) 4675

Blümel R., Lukacs R., Zimmermann B., Bağcıoğlu M., Kohler A.
Observation of Mie ripples in the synchrotron FTIR spectra of spheroidal pollen grains
Journal of the Optical Society of America A 35 (2018) 1769

Zimmermann B., Bağcıoğlu M., Sandt C., Kohler A.
Vibrational microspectroscopy enables chemical characterization of single pollen grains as well as comparative analysis of plant species based on pollen ultrastructure
Planta 242 (2015) 1237

Lukacs R., Blümel R., Zimmermann B., Bağcıoğlu M., Kohler A.
Recovery of absorbance spectra of micrometer-sized biological and inanimate particles
Analyst 140 (2015) 3273-3284

Published 25. February 2021 - 12:56 - Updated 30. June 2021 - 16:07