Project No: 18207701 and 19107969
Period covered - start date: 2018
Period covered - end date: 2019
Projects Administrator: Boris Zimmermann
Collaboration: Humboldt University of Berlin and Norwegian University of Life Sciences
HUB collaboration: Janina Kneipp
Depending on the sample preparation and spatial resolution chosen for an FTIR experiment, the selectivity of the spectroscopic method can vary greatly. For a comprehensive characterization of phenotype differences in grass pollen, which do not show speciesspecific morphology, adjustable selectivity for specific microscopic pollen structures are needed. The projects will assess the specificity and selectivity of FTIR microscopic probing as a function of lateral resolution. Single pollen grains from different grass species will be studied. To validate the spatial selectivity in the pollen samples, the FTIR microspectra at different spot sizes will be compared with the chemical information obtained by MALDI-TOF MS in different matrix/extraction protocols. The experiments can provide an improved classification and characterization of the specific chemical composition of pollen, which is of relevance for diverse applications, ranging from allergology over climate research to agriculture.
Diehn S., Zimmermann B., Tafintseva V., Bağcıoğlu M., Kohler A., Ohlson M., Fjellheim S., Kneipp J.
Discrimination of grass pollen of different species by FTIR spectroscopy of individual pollen grains.
Analytical and Bioanalytical Chemistry 412 (2020) 6459-6474