Norwegian Research Council - FMETEKN Grant

Project number: 257622
Period covered - start date: 2016
Period covered - end date: 2024
Project's coordinator: Duncan Akporiaye, SINTEF
SP3 leader: Vincent Eijsink, NMBU
WP3.4 leader: Alexander Wentzel, SINTEF


Bio4Fuels for the first time brings together leading Norwegian research institutions, universities, key national and international partners, major forestry resources owners and regional authority into one common initiative on production of energy carriers from renewable sources. The project aims to develop innovative technology and support industries to realize economic and sustainable conversion of lignocellulosic  biomass and organic residues to transportation fuels, along with added value chemicals, heat and power. According to the IEA-roadmap, 20-30% of global energy demand could be supplied from the conversion of biomass.

Recently, the use of oleaginous microorganisms for production of lipids (single cell oils) has emerged as a novel route for biodiesel production and as an alternative source of polyunsaturated fatty acids. Oleaginous fungi have the ability to utilize lignocellulosic biomass as a carbon source, and may accumulate up to 80 % (w/w) lipids. Some oleaginous fungi produce oils that are rich in high price PUFAs (polyunsaturated fatty acids) with a market value of up to 150 US $ per kg for the pharmacy, feed and food industry. The BioSpec group will use the lignocellulose hydrolysates, produced at the Faculty of Chemistry, Biotechnology and Food Science, as a carbon source for the production of high value single cell oils and other byproducts by oleaginous filamentous fungi.




Dzurendova S., Zimmermann B., Kohler A., Reitzel K., Nielsen U.G., Dupuy--Galet B.X., Leivers S., Horn S.J., Shapaval V.
Calcium affects polyphosphate and lipid accumulation in Mucoromycota fungi
Journal of Fungi 7 (2021) 300

Langseter A.M., Dzurendova S., Shapaval V., Kohler A., Ekeberg D., Zimmermann B.
Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi
Microbial Cell Factories 20 (2021) 59

Slaný O. , Klempová T., Shapaval V., Zimmermann B., Kohler A., Čertík M.
Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation
Microorganisms 9 (2021) 170

Dzurendova S., Zimmermann B., Tafintseva V., Kohler A., Horn S.J., Shapaval V.
Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides
Journal of Fungi 6 (2020) 260

Slaný O., Klempová T., Shapaval V., Zimmermann B., Kohler A., Čertík M.
Biotransformation of Animal Fat-By Products into ARA-Enriched Fermented Bioproducts by Solid-State Fermentation of Mortierella alpina 
Journal of Fungi 6 (2020) 236.

Dzurendova S., Zimmermann B., Tafintseva V., Kohler A., Ekeberg D., Shapaval V.
The influence of phosphorus availability and the nature of nitrogen on the biomass production and accumulation of lipids in oleaginous Mucoromycota fungi
Applied Microbiology and Biotechnology 104 (2020) 8065

Dzurendova S., Zimmermann B., Kohler A., Tafintseva V., Slany O., Certik M., Shapaval V.
Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi
PLoS ONE 15 (2020) e0234870

Kosa G., Vuoristo K., Horn S.J., Zimmermann B., Afseth N.K., Kohler A., Shapaval V.
Assessment of the scalability of a microtiter plate system for screening of oleaginous microorganisms.
Applied Microbiology and Biotechnology 102 (2018) 4915.

Kosa G., Zimmermann B., Kohler A., Ekeberg D., Afseth N.K., Mounier J., Shapaval V.
High-throughput screening of Mucoromycota fungi for production of low- and high value lipids.
Biotechnology for Biofuels 11:66 (2018)

Kosa G., Shapaval V., Kohler A., Zimmermann B.
FTIR spectroscopy as a unified method for simultaneous analysis of intra- and extracellular metabolites in high-throughput screening of microbial bioprocesses.
Microbial Cell Factories 16:195 (2017).


Published 2. June 2018 - 22:12 - Updated 28. April 2021 - 15:02