Publications by the BioRef group


Agboola, J. O., Schiavone, M., Øverland, M., Morales-Lange, B., Lagos, L., Arntzen, M. Ø., Lapeña, D., Eijsink, V.G.H, Horn, S.J.H., Mydland, L.T., François, J. M., & Hansen, J. Ø. (2021). Impact of down-stream processing on functional properties of yeasts and the implications on gut health of Atlantic salmon (Salmo salar). Scientific Reports, 11, 4496.

Published online: 24.02.2021


Jameson, J. K., Mathiesen, G., Pope, P. B., Westereng, B., & La Rosa, S. L. (2021). Biochemical characterization of two cellobiose 2-epimerases and application for efficient production of lactulose and epilactose. Current Research in Biotechnology.

Published online: 23.02.2021


Kadić, A., Várnai, A., Eijsink, V. G., Horn, S. J., & Lidén, G. (2021). In situ measurements of oxidation–reduction potential and hydrogen peroxide concentration as tools for revealing LPMO inactivation during enzymatic saccharification of cellulose. Biotechnology for Biofuels, 14, 46.

Published online: 18.02.2021


Arntzen, M. Ø., Pedersen, B., Klau, L. J., Stokke, R., Oftebro, M., Antonsen, S. G., Fredriksen, L., Sletta, H., Aachmann, F.L., Horn, S.J. & Eijsink, V. G. (2021). Insights into alginate degradation through the characterization of a thermophilic exolytic alginate lyase. Applied and Environmental Microbiology, 87:e02399-20.

Published online: 04.01.2021



2020 (16 papers)


Wahid, R. & Horn, S. J. (2020) The effect of mixing rate and gas recirculation on biological CO2 methanation in two-stage CSTR systems, Biomass and Bioenergy,


Michalak, L., Gaby, J. C., Lagos, L., La Rosa, S. L., Hvidsten, T. R., Tétard-Jones, C., ... & Dröge, J. (2020). Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut. Nature communications, 11(1), 5773


Svennevik, O.K., Jonassen, K.R., Svensson, K. et al. (2020). Protecting Thermally Hydrolyzed Biosolids from Pathogenic Bacterial Growth by Addition of Compost. Waste Biomass Valor,


Solberg, B., Moiseyev, A., Hansen, J. Ø., Horn, S. J., & Øverland, M. (2020). Wood for food: Economic impacts of sustainable use of forest biomass for salmon feed production in Norway. Forest Policy and Economics, 122, 102337.



Dzurendova, S., Zimmermann, B., Tafintseva, V., Kohler, A., Horn, S.J., & Shapaval, V. (2020). Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides. J. Fungi, 6, 260.



Østby, H., Hansen, L. D., Horn, S. J., Eijsink, V. G., & Várnai, A. (2020). Enzymatic processing of lignocellulosic biomass: principles, recent advances and perspectives. Journal of Industrial Microbiology & Biotechnology, 1-35.


Westereng, B., Kracun, S. K., Leivers, S., Arntzen, M., Aachmann, F. L., & Eijsink, V. G. (2020). Synthesis of Glycoconjugates Utilizing the Regioselectivity of a Lytic Polysaccharide Monooxygenase. Scientific Reports 10, 13197


Lagos, L., Leanti La Rosa, S., Ø Arntzen, M., Ånestad, R., Terrapon, N., Gaby, J. C., & Westereng, B. (2020). Isolation and Characterization of Extracellular Vesicles Secreted In Vitro by Porcine Microbiota. Microorganisms, 8(7), 983.


Dysvik, A., La Rosa, S. L., De Rouck, G., Rukke, E. O., Westereng, B., & Wicklund, T. (2020). Microbial dynamics in traditional and modern sour beer production. Applied and Environmental Microbiology, 86:e00566-20


Kristoffersen, K. A., van Amerongen, A., Böcker, U., Lindberg, D., Wubshet, S. G., de Vogel-van den Bosch, H., Horn, S.J.  & Afseth, N. K. (2020). Fourier-transform infrared spectroscopy for monitoring proteolytic reactions using dry-films treated with trifluoroacetic acid. Scientific Reports, 10, 7844


Svennevik, O. K., Nilsen, P. J., Solheim, O. E., Westereng, B., & Horn, S. J. (2020). Quantification of soluble recalcitrant compounds in commercial thermal hydrolysis digestates. Water Environment Research.


Costa, T. H., Kadic', A., Chylenski, P., Várnai, A., Bengtsson, O., Lidén, G., Eijsink, V.G.H & Horn, S. J. (2020). Demonstration‐scale enzymatic saccharification of sulfite‐pulped spruce with addition of hydrogen peroxide for LPMO activation. Biofuels, Bioproducts and Biorefining, 14(4), 734-745.


Michalak, L., La Rosa, S. L., Leivers, S., Lindstad, L. J., Røhr, Å. K., Aachmann, F. L., & Westereng, B. (2020). A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex β-mannans. Proceedings of the National Academy of Sciences, 117(13), 7122-7130.


Dysvik, A., La Rosa, S. L., Liland, K. H., Myhrer, K., Østlie, H. M., De Rouck, G., Rukke, E. O., Westereng, B. & Wicklund, T. (2020). Co-fermentation involving Saccharomyces cerevisiae and Lactobacillus species tolerant to brewing-related stress factors for controlled and rapid production of sour beer. Frontiers in Microbiology, 11, 279.


Lapeña, D., Kosa, G., Hansen, L. D., Mydland, L. T., Passoth, V., Horn, S. J., & Eijsink, V. G. (2020). Production and characterization of yeasts grown on media composed of spruce-derived sugars and protein hydrolysates from chicken by-products. Microbial Cell Factories, 19(1), 19.


Sørlie, M., Horn, S. J., Vaaje-Kolstad, G., & Eijsink, V. G. (2020). Using chitosan to understand chitinases and the role of processivity in the degradation of recalcitrant polysaccharides. Reactive and Functional Polymers, 12, 104488.






2019 (15 papers)


David Lapeña, Pernille M. Olsen, Magnus Ø. Arntzen, Gergely Kosa, Volkmar Passoth, Vincent G. H. Eijsink, Svein J. Horn, 2020, Spruce sugars and poultry hydrolysate as growth medium in repeated fed-batch fermentation processes for production of yeast biomass, Bioprocess Biosyst Eng, 43, 723–736.


Dysvik, A., La Rosa, S. L., Buffetto, F., Liland, K. H., Myhrer, K. S., Rukke, E. O., Wicklund, T. & Westereng, B. (2020). Secondary lactic acid bacteria fermentation with wood-derived xylooligosaccharides as a tool to expedite sour beer production. Journal of Agricultural and Food Chemistry, 68(1), 301-314



Costa, T. H. F., Eijsink, V. G., & Horn, S. J. (2019). The use of lytic polysaccharide monooxygenases in anaerobic digestion of lignocellulosic materials. Biotechnology for Biofuels, 12(1), 270.



Svennevik, O. K., Solheim, O. E., Beck, G., Sørland, G. H., Jonassen, K. R., Rus, E., Westereng, B., Horn, S. J., Higgins, M. J. & Nilsen, P. J. (2019). Effects of post anaerobic digestion thermal hydrolysis on dewaterability and moisture distribution in digestates. Water Science and Technology, 80 (7), 1338–1346.



Dysvik, A., Liland, K. H., Myhrer, K. S., Westereng, B., Rukke, E. O., De Rouck, G., & Wicklund, T. (2019). Pre‐fermentation with lactic acid bacteria in sour beer production. Journal of the Institute of Brewing, 125 (3), 342-356.



Kristoffersen, K. A., Liland, K. H., Böcker, U., Wubshet, S. G., Lindberg, D., Horn, S. J., & Afseth, N. K. (2019). FTIR-based hierarchical modeling for prediction of average molecular weights of protein hydrolysates. Talanta, 205, 120084.



Wahid, R., Mulat, D. G., Gaby, J. C., & Horn, S. J. (2019). Effects of H 2: CO 2 ratio and H 2 supply fluctuation on methane content and microbial community composition during in-situ biological biogas upgrading. Biotechnology for Biofuels, 12(1), 104.



Svennevik, O. K., Beck, G., Rus, E., Westereng, B., Higgins, M., Solheim, O. E., Nilsen, P.J. & Horn, S. J. (2019). CNash-A novel parameter predicting cake solids of dewatered digestates. Water Research, 158, 350-358.



Chylenski, P., Bissaro, B., Sørlie, M., Røhr, Å. K., Várnai, A., Horn, S. J., & Eijsink, V. G. (2019). Lytic Polysaccharide Monooxygenases in Enzymatic Processing of Lignocellulosic Biomass. ACS Catalysis, 9, 4970-4991.


Aarum, I., Solli, A., Gunnarsson, H., Kalyani, D., Devle, H., Ekeberg, D., & Stenstrøm, Y. (2019). Comparison of pyrolyzed lignin before and after milled wood lignin purification of Norway spruce with increasing steam explosion. Wood Science and Technology, 1-18.



La Rosa, S. L., Leth, M. L., Michalak, L., Hansen, M. E., Pudlo, N. A., Glowacki, R., Pereira, G., Workman, C. T., Arntzen, M. Ø., Pope, P. B., Martens, E. C. Hachem, M. A., & Westereng, B. (2019). The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans. Nature communications, 10, 905.



Vuoristo, K. S., Fredriksen, L., Oftebro, M., Arntzen, M. Ø., Aarstad, O. A., Stokke, R., Steen, I. H., Hansen, L. D., Schüller, R. B., Aachmann, F. L, Horn, S. J., & Vincent G. H. (2019). Production, characterization and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic Mid-Ocean Ridge. Journal of agricultural and food chemistry. 67 (10), pp 2936–2945.


La Rosa, S. L., Kachrimanidou, V., Buffetto, F., Pope, P. B., Pudlo, N. A., Martens, E. C., Rastall, R. A., Gibson, G. R., & Westereng, B. (2019). Wood-Derived Dietary Fibers Promote Beneficial Human Gut Microbiota. mSphere, 4(1), e00554-18.



Fredriksen, L., Stokke, R., Jensen, M. S., Westereng, B., Jameson, J. K., Steen, I. H., & Eijsink, V. G. H. (2019). Discovery of a thermostable GH10 xylanase with broad substrate specificity from the Arctic Mid-Ocean Ridge vent system. Appl. Environ. Microbiol., 85(6), e02970-18.




2018 (14 papers)


Michalak, L., Knutsen, S. H., Aarum, I., & Westereng, B. (2018). Effects of pH on steam explosion extraction of acetylated galactoglucomannan from Norway spruce. Biotechnology for biofuels, 11(1), 311.



Lapeña, D., Vuoristo, K. S., Kosa, G., Horn, S. J., & Eijsink, V. G.H. (2018). A comparative assessment of enzymatic hydrolysis for valorization of different protein-rich industrial by-products. Journal of Agricultural and Food Chemistry. 66 (37), pp 9738–9749.



Müller, G., Chylenski, P., Bissaro, B., Eijsink, V.G.H, & Horn, S.J. (2018). The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail. Biotechnology for Biofuels, 11:209



Sharma, S., Hansen, L. D., Hansen, J. Ø., Mydland, L. T., Horn, S. J., Øverland, M., Eijsink, V.G.H & Vuoristo, K. S. (2018). Microbial protein produced from brown seaweed and spruce wood as a feed ingredient. Journal of agricultural and food chemistry. 66 (31), 8328–8335.



Ida Aarum , Hanne Devle, Dag Ekeberg, Svein J. Horn, Yngve Stenstrøm (2018) Characterization of Pseudo-Lignin from Steam Exploded Birch, ACS Omega, 3 (5), 4924–4931


Liu, B., Kognole, A. A., Wu, M., Westereng, B., Crowley, M. F., Kim, S., Dimarogona, M., Payne, C. M., & Sandgren, M. (2018). Structural and molecular dynamics studies of a C1‐oxidizing lytic polysaccharide monooxygenase from Heterobasidion irregulare reveal amino acids important for substrate recognition. The FEBS journal, 285(12), 2225-2242.


Kosa, G., Vuoristo, K.S., Horn, S.J., Zimmermann, B., Afseth, N.K., Kohler, A., Shapaval, V. (2018) Assessment of the scalability of a microtiter plate system for screening of oleaginous microorganisms. Applied Microbiology and Biotechnology, 102 (11), 4915-4925.



Dragicevic, I., Eich-Greatorex, S., Sogn, T. A., Horn, S. J., & Krogstad, T. (2018). Use of high metal-containing biogas digestates in cereal production-Mobility of chromium and aluminium. Journal of environmental management, 217, 12-22.



Leth, M.L., Ejby, M., Workman, C., Ewald, D.A., Pedersen, S.S., Sternberg, C., Bahl, M.I., Licht, T. R., Aachmann, F.L., Westereng, B., & Abou Hachem, M. (2018). Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut. Nature Microbiology,



Sharma, S., Neves, L., Funderud, J., Mydland, L. T., Øverland, M., & Horn, S. J. (2018). Seasonal and depth variations in the chemical composition of cultivated Saccharina latissima. Algal Research, 32, 107-112.



Mulat, D. G., Dibdiakova, J., & Horn, S. J. (2018). Microbial biogas production from hydrolysis lignin: insight into lignin structural changes. Biotechnology for biofuels, 11(1), 61.



Solli, L., Schnürer, A, & Horn, S. J. (2018). Process performance and population dynamics of ammonium tolerant microorganisms during co-digestion of fish waste and manure. Renewable Energy, 125, 529-536.



Mulat, D. G., Huerta, S. G., Kalyani, D., & Horn, S. J. (2018). Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii. Biotechnology for biofuels, 11(1), 19.



Svensson, K., Kjørlaug, O., Higgins, M. J., Linjordet, R., & Horn, S. J. (2018). Post-anaerobic digestion thermal hydrolysis of sewage sludge and food waste: Effect on methane yields, dewaterability and solids reduction. Water research, 132, 158-166.




2017 (15 papers)




Zamanzadeh, M., Hagen, L. H., Svensson, K., Linjordet, R., & Horn, S. J. (2017). Biogas production from food waste via co-digestion and digestion-effects on performance and microbial ecology. Scientific reports, 7(1), 17664.



Gaby, J. C., Zamanzadeh, M., & Horn, S. J. (2017). The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste. Biotechnology for biofuels, 10(1), 302.



Vivekanand, V., Mulat, D. G., Eijsink, V. G., & Horn, S. J. (2018). Synergistic effects of anaerobic co-digestion of whey, manure and fish ensilage. Bioresource technology, 249, 35-41.



Greses, S., Gaby, J. C., Aguado, D., Ferrer, J., Seco, A., & Horn, S. J. (2017). Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions. Algal Research, 27, 121-130.



Bissaro, B., Røhr, Å. K., Müller, G., Chylenski, P., Skaugen, M., Forsberg, Z., Horn, S.J., Vaaje-Kolstad, G., & Eijsink, V. G.H. (2017). Oxidative cleavage of polysaccharides by monocopper enzymes depends on H 2 O 2. Nature chemical biology, 13(10), 1123-1128.



Ravanal, M. C., Sharma, S., Gimpel, J., Reveco-Urzua, F. E., Øverland, M., Horn, S. J., & Lienqueo, M. E. (2017). The role of alginate lyases in the enzymatic saccharification of brown macroalgae, Macrocystis pyrifera and Saccharina latissima. Algal Research, 26, 287-293.



Aarum, I., Devle, H., Ekeberg, D., Horn, S. J., & Stenstrøm, Y. (2017). The effect of flash pyrolysis temperature on compositional variability of pyrolyzates from birch lignin. Journal of Analytical and Applied Pyrolysis, 127, 211-222.



Chylenski, P., Petrović, D. M., Müller, G., Dahlström, M., Bengtsson, O., Lersch, M., Siika-aho, M., Horn, S.J., & Eijsink, V. G.H. (2017). Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs. Biotechnology for biofuels, 10(1), 177.



Kosa, G., Kohler, A., Tafintseva, V., Zimmermann, B., Forfang, K., Afseth, N. K.,  Tzimorotas, D., Vuoristo, K.S., Horn, S.J., Mounier, J., & Shapaval, V. (2017). Microtiter plate cultivation of oleaginous fungi and monitoring of lipogenesis by high-throughput FTIR spectroscopy. Microbial cell factories, 16(1), 101.



Kalyani, D. C., Fakin, T., Horn, S. J., & Tschentscher, R. (2017). Valorisation of woody biomass by combining enzymatic saccharification and pyrolysis. Green Chemistry, 19(14), 3302-3312.!divAbstract



Liu, Z., Gay, L. M., Tuveng, T. R., Agger, J. W., Westereng, B., Mathiesen, G., Horn, S.J., Vaaje-Kolstad, G., van Aalten, D.M.F, & Eijsink, V. G. H. (2017). Structure and function of a broad-specificity chitin deacetylase from Aspergillus nidulans FGSC A4. Scientific Reports, 7(1), 1746.



Mravec, J., Kračun, S. K., G Rydahl, M., Westereng, B., Pontiggia, D., De Lorenzo, G., Domozych, D.S., & Willats, W. G. (2017). Oligogalacturonide‐derived molecular probe demonstrates the dynamics of calcium‐mediated pectin complexation in cell walls of tip‐growing structures. The Plant Journal, 91(3), 534-546.



Svensson, K., Kjørlaug, O., Horn, S. J., & Agger, J. W. (2017). Comparison of approaches for organic matter determination in relation to expression of bio-methane potentials. Biomass and Bioenergy, 100, 31-38.



Kalyani, D. C., Zamanzadeh, M., Müller, G., & Horn, S. J. (2017). Biofuel production from birch wood by combining high solid loading simultaneous saccharification and fermentation and anaerobic digestion. Applied Energy, 193, 210-219.



Chylenski, P., Forsberg, Z., Ståhlberg, J., Várnai, A., Lersch, M., Bengtsson, O., Sæbø, S., Horn, S.J., & Eijsink, V. G. H. (2017). Development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass. Journal of biotechnology, 246, 16-23.



2016 (7 papers)


Kojima, Y., Várnai, A., Ishida, T., Sunagawa, N., Petrovic, D. M., Igarashi, K., Jellison, J., Goodell, B., Alfredsen, G., Westereng, B., Eijsink, V. G.H., & Yoshida, M. (2016). A lytic polysaccharide monooxygenase with broad xyloglucan specificity from the brown-rot fungus Gloeophyllum trabeum and its action on cellulose-xyloglucan complexes. Applied and environmental microbiology, 82(22), 6557-6572.


Nekiunaite, L., Petrović, D. M., Westereng, B., Vaaje‐Kolstad, G., Hachem, M. A., Várnai, A., & Eijsink, V. G. (2016). FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern. FEBS letters, 590(19), 3346-3356.


Puchart, V., Agger, J. W., Berrin, J. G., Várnai, A., Westereng, B., & Biely, P. (2016). Comparison of fungal carbohydrate esterases of family CE16 on artificial and natural substrates. Journal of biotechnology, 233, 228-236.


Zamanzadeh, M., Hagen, L. H., Svensson, K., Linjordet, R., & Horn, S. J. (2016). Anaerobic digestion of food waste–Effect of recirculation and temperature on performance and microbiology. Water Research, 96, 246-254.


Westereng, B., Arntzen, M. Ø., Aachmann, F. L., Várnai, A., Eijsink, V. G., & Agger, J. W. (2016). Simultaneous analysis of C1 and C4 oxidized oligosaccharides, the products of lytic polysaccharide monooxygenases acting on cellulose. Journal of Chromatography A, 1445, 46-54.


Sharma, S., & Horn, S. J. (2016). Enzymatic saccharification of brown seaweed for production of fermentable sugars. Bioresource technology, 213, 155-161.



Frank, J. A., Arntzen, M. Ø., Sun, L., Hagen, L. H., McHardy, A. C., Horn, S. J., Eijsink, V.G.H., Schnürer, A. & Pope, P. B. (2016). Novel syntrophic populations dominate an ammonia-tolerant methanogenic microbiome. MSystems, 1(5), e00092-16.




Published 29. March 2016 - 9:01 - Updated 3. March 2021 - 11:52