Course code KJB311

KJB311 Protein NMR

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Showing course contents for the educational year 2015 - 2016 .

Course responsible: Lars Skjeldal
ECTS credits: 5
Faculty: Department of Chemistry, Biotechnology and Food Science
Teaching language: EN, NO
(NO=norsk, EN=Engelsk)
Limits of class size:
Minimum 5, maximum 50 students.
Teaching exam periods:
08 August block. Teaching and evaluation in August. 
Course frequency: Annually.
First time: Study year 2014-2015
Course contents:
An introduction to literature regarding one-dimensional and two-dimensional protein NMR is presented. Students will get familiar with handling proteins in solutions including deuterated solvents. Pulssequences from the litterature will be discussed (some NMR experience is needed.)
Further, this course includes assignments of two-dimensional spectra and the use of isotope incorporation. At the end of this course, the calculation of a three-dimensional protein structure based upon NMR spectra is presented.
Learning outcome:
The handling of proteins in solution in order to do NMR. Assigning amino acids i a two-dimensional proton-proton TOCSY spectrum. Knowledge about calculating a 3D protein structure.
Learning activities:
Selected publications.Lecture notes.The use of relevant software.
Teaching support:

E-mail mailto:lars.skjeldal@nmbu.nolars.skjeldal@nmbu.no

Fronter. 

Syllabus:
Relevant publications. 
Prerequisites:
KJM311 Organic Spectroscopy (or equivalent).KJB310 Protein Chemistry (or equivalent).

Recommended prerequisites:
Assessment:
3,5 hours written exam, counts 100 %.
Nominal workload:

150 hours in total, intensively over three weeks:

  • 14 hrs lectures
  • 24 hrs lections with computer
  • 112 hrs self study
Entrance requirements:
Special requirements in science.
Type of course:
Over three weeks:
Lectures 14 hours
Lections with computer 24 hours
Examiner:
An external examiner marks 25 randomly selected exam papers. 
Allowed examination aids: No calculator, no other aids
Examination details: One written exam: A - E / Ikke bestått