BIO350 RNA in situ RNA Hybridization Techniques
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Showing course contents for the educational year starting in 2020 .
Course responsible: Trine Hvoslef-Eide
Teachers: Tone Ingeborg Melby
ECTS credits: 5
Faculty: Faculty of Biosciences
Teaching language: EN, NO
Limits of class size:
Teaching exam periods:
This course runs in the January semester in the Plant Cell Laboratory in and have a heavy time table for the first two weeks.
Course frequency: Annually
First time: 2007H
Master´s degree students in Plant Science, Biology or Biotechnology, as well as PhD students.
The course consists of lectures to give the theory, but mostly practicals to illustrate the whole process. The students can bring their own biological material to the first day of class, in agreement with the teacher, so that preparations can be made. The students have to hand in a lab journal that has to be approved.
The students shall be able to harvest their plant or animal material, fix it, wax-embed it, section it in a microtome, transfer to a pre-coated slide, make probes of potentially interesting gene sequences, hybridise with the probes, develop and examine under the microscope. One is able to see which genes are active (expressed) at any given time in a developmental process. The students should be able to see the potential and the limitations for the technique in plant sciences.
Lectures, laboratory work.
Lectures, web support through Canvas and guidance during exercises in the laboratory.
Practical in situ hybridisation, by Trude Schwartzacher and Pat Heslop-Harrison (2002). The book is no longer available from the publishers, so we have copied the relevant chapter for use on Canvas for our students
Knowledge of molecular biology
Practicals in the laboratory and journal
Submitted laboratory report counts 100%.
Ca. 150 hours.
Special requirements in Science
Reduction of credits:
Type of course:
There will normally be activities from 0830am-4pm each day for the first 2 weeks of the January semester, with some modifications depending on how much time is spent each day. We follow the protocols for RNA in situ hybridization.
This course is meant for undergraduate and PhD students who work on gene expression and wish to learn techniques to study gene expression in anatomical sections of plants or animals. The students can bring their own plant material if they wish. We will fix the samples, embed them in wax, section, and hybridise with their own probes or probes provided in the course. Lastly, we will develop the expression and examine the result under the microscope.
The examiner takes part in the assessment.
Examination details: Continuous exam: Passed / Failed