- Genetikk, evolusjon og planteproduksjon
Experiences
- Positions & international training
NMBU: Post doc (1993/94 & 1996-98), ass. professor (1998-2004), professor 2004-
ENS/Lyon, France: Post doc 1994-96
UC-Berkeley: Sabbatical 2004/5 & 2019
Yale University: Sabbatical 2012/13
COST actions: WG leader on communication & MC member in 5 on biotechnology
- Teaching
Introductory course genetics (BIO120) ~200 students, 1988-2018 with a few leaves
Introductory course in cell biology (BIO100) ~200 students, 2018-2020
Developmental biology MSc and PhD (BIO320, BIO420) since course developed 1996-
Involved in BIO101 & HFX133.
- Student guidance
PhD student 2019- Ioannis Theodorou (Previous Lene Therese Olsen + co-supervisor on several)
Masterstudents Current 2022- Espen Evju (Plant Biology/Biotechnology)
Iva Andrasevic (Plant Biology/Biotechnology)
Dorota Monika Jaskula (Biotechnology/Bioinformatics)
- Project collabortions
-Sorbonne/CNRS Roscoff: Cell patterning/development Saccharina latissima (PUZZLE)
https://www.sb-roscoff.fr/en/charrier-benedicte/509
-NMBU LANDSAM (landscape design)/HH (business school)/BIOVIT on Sugar kelp. Fjærelandskap.
-NIBIO: Green CRISPR Antiviral Leaves (GC-AVL) & Green Antiviral Lettuce (G-AVL) (Avl = Breeding)
https://www.nibio.no/ansatte/jihong-liu-clarke
-Meristem identity and development
https://plantandmicrobiology.berkeley.edu/profile/fletcher
https://www.ars.usda.gov/people-locations/person?person-id=1756
-Submitted EU proposal Sea2Biodive4Life on biodiversity using kelp genomics with 7 European partners (SE, DK, NL, FR, IE, PT, NO) + Morocco, Brazil & China
https://www.sb-roscoff.fr/en/cock-j-mark/14
- Science
I started by identifying genes involved in seed development and cell identity, screening gene collections to isolate genes crucial to function and gene regulation for identity of epidermal cells (DEK11, LTP2, Des52), identified the ESR genes3,4 part of the CLE (CLV3/ESR) gene family5,6and showed how GASA47 and DEK18 are crucial for meristem identity and functions in plants. My interests have shifted to studying development of sugar kelp, exciting since science of macroalgae is lagging far behind land plants and animals9. We have followed single cell divisions from zygote through young embryos10 and are currently developing a model explaining early developmental control and possibly flexibility. This will be combined with the first transcriptomic mapping of this development, while the annotated full genome sequence is expected published soon (Mark Cock pers. com. 2019-21). We have used and given webinars on techniques such as microarray&RNAseq, marker studies for gene regulation11, RNAi12 and Genome editing13. On the applied side I collaborate with Jihong Liu Clarke on antibody production for oral vaccinations from lettuce and planned basic studies including genome editing in tobacco plants. For complete ref list also including YouTube videos for dissemination see Cristin.
Ref
1)Lid SE,..Opsahl-Ferstad HG.. (2004) The maize .. dil1, dil2.. lack control of the mitotic division plane ..in the aleurone.. Planta 218:370-8
2) Olsen L,..,Opsahl-Sorteberg (2008) ..des5.. seed development and HvDek1, HvCr4, and HvSal1 gene regulation. J Exp Bot 59: 3753-65
3)Opsahl-Ferstad HG,.., Rogowsky P (1997) ZmEsr, a novel endosperm .. gene expressed in a restricted region around the maize embryo. Pl J 12:235-46
4) Bonello JF, Opsahl-Ferstad HG et al. (2000) Esr genes show different levels of expression in the same region of maize endosperm. Gene 246:219-227
5) Cock JM & McCormick S (2001) A large family of genes that share homology with CLAVATA3. Plant Physiol. 126:939-42
6) Hirakawa et al. (2019) Control of proliferation in the haploid meristem by CLE peptide signaling in Marchantia polymorpha. PLoS Genet 15: e1007997
7) Roxrud I, , Opsahl-Sorteberg HG (2007) GASA4,.., regulates flowering and seed development. Plant Cell Physiol. 48:471-83
8) Liang Z, Brown RC, Fletcher JC, Opsahl-Sorteberg HG (2015) Calpain-Mediated Positional Information Directs Cell Wall Orientation to Sustain Plant Stem Cell Activity, Growth and Development. Plant Cell Physiol. 56:1855-66
9) Theodorou I, Kovi MR, Liang Z, Opsahl-Sorteberg (2022) CHP33: Genetic and genomic approaches for improved and sustainable brown algal cultivation In: Sustainable Global Resources of Seaweeds Vol2 Ed: Gokare A Ravishankar and Ambati Ranga Rao. Springer In press
10) Theodorou I, Opsahl-Sorteberg HG, Charrier B (2021) Preparation of zygotes and embryos.S. latissima.Bio-101: e4132. DOI: 10.21769/BioProtoc.4132.
11) Liang Z & Opsahl-Sorteberg HG (2019) Use of the β-Glucuronidase (GUS) ..Reporter System to Localize Promoter Activities..DEK1. Methods Mol Biol
12) Clauvis..Sorteberg..(2020) RNA-based biocontrol compounds: current status and perspectives to reach the market. Pest Manag Sci. 76:841-45
13) Dragana M,..,Opsahl-Sorteberg HG,.. (2021) Targeted plant improvement through genome editing: from laboratory to field. Plant Cell Rep. 40: 935-951