Plant Cell Lab - Plantecellelaboratoriet

The Plant Cell Laboratory at Biovit is a research laboratory for the research and teaching done by the plant biology research groups.

Kontaktinfo

E-post: tone.melby@nmbu.no

Telefon: 67232836

Besøk- og postadresser

Åpningstider

Kl 08:00 - 15:45

15. mai - 14. september:
Kl 08:00 - 15:00

Welcome to work in the Plant Cell Lab

Contact: Lab. coordinator Tone I. Melby, tone.melby@nmbu.no

The Plat Cell Lab is located at Faculty of Biosciences

Field of work and laboratory analysis

Cell- and tissue culture (solid- and liquid cultures, bioreactors)
Molecular biology (gene sequences and gene transcript sequences, analyses of gene expression, copy number variation (CNV), CPD-DNA, in situ hybridization, and other standard molcular work)
Protein (Western-blot analyses, ELISA)
Cell biology ( preparation for microscopy, immunolocalisation, light microscopy)
Biochemistry analysis (carbohydrates, enzymes (Catalase, SOD etc), antioxidant, pigments and other metabolites)
Transgenic organisms (create and working with GMOs, gene editing/CRISPR)

The Plant Cell Lab is used for teaching several student courses in plant biotechnology. These are 200 and 300 courses that depend on the facilities and instruments in the laboratory.
The master and PhD students use the lab for their projects in plant biotechnology and plant biology.
The lab is also used for preparation for student courses in plant physiology and genetics.
Three courses have all the lab exercises in the lab.
- BIO244 Biotechnology in Plants. Spring semester
- BIO350 In situ RNA hybridization. January block
- BIO327 From gene to function in plants. Autumn semester
- Preparing for the lab courses
BOT130, BOT200 Plant Physiology. Autumn semester
Master students
PhD students

Instruments and equipments

Equipment for making nutrient media.
Autoclave etc
Sterile rooms (4)
Laminair air flow benches (LAF) (8) and LAF Biohazard bench (1)
Growt rooms for cell- and tissue cultures(3)
Light 20°C, Light 23°C, Dark 23°C
Bioreactors (6)
6 x 2 liters
Light Microscopes
Leica DM 5000B fluorescens microscope with camera (Leica DFC 425) connected to a computer with image analysis software (LAS), manuell multifocus.
Fluorescens filters: UV (A), Blue(I3), Green(N2
Two old microscope, upright (1) and invert (1) for liquid tissue culture
Stereo Microscopes
Leica M205 with automatic multifocus and camera/software like DM 5000B.
Leica MZFLIII fluorescens with camera (Leica DC 300F) connected to a computer with image analysis software.
Fluorescens filters: UV, GFP2(Plus fluorescens), GFP3 (Plant fluorescens)
Routine manual stereo microscopes (10)
TissueLyser II. Rapid Cell & Tissue Homogenization
DNA/RNA UV-cleaner. PCR-cabinet for master-mix.
DNA gel electrophoresis equipment
Bioanalyzer
Aglient 2100 bioanalyzer. Check the quality of RNA/DNA, also the quantity.
Bioanalyzer
(Photo: Aglient Technologies)
PCR systems
Quantitative PCR. 7500 Fast Real-Time PCR System, Applied Biosystem
C1000 Touch Thermal Cycler, Biorad. End-point PCR, capabilities to run gradient PCR.
T100 Thermal Cycler. End-point PCR, capabilities to run gradient PCR (2)
QPCR
(Photo: Thermo Fisher Scientific)
QX200 Droplet Digital PCR (ddPCR), BioRad
Applications like Gene Expression, CRISPR analysis,
Copy number variation (CNV), rare event/mutation detection
ddPCR
(Photo: Bio Rad Laboratories)
Proteins
Protein Electrophoresis. Mini-PROTEAN systems, Bio-Rad.
Western Blot. Trans-Blot Turbo Transfer system, Bio-Rad. Rapid transfer.
Imaging systems
Chemi-Doc MP, Bio-Rad. Gel or western blot imaging. Multiplex fluorescent western blotting, chemiluminiscence detection, geldocumentation, stain-free tecnology.
Chemi-Doc MP
(Photo: Bio Rad Laboratories)
Fluorometer
Qubit assays, Life. Accurately quantifies DNA, RNA and protein.
Need assay kits, standards.
Qubit
Foto: Life Technologies
Multiscan SkyHigh Microplate Reader, ThermoFisher.
200-1000 nm. Plates, cuvette and µDrop Duo Plates (2-10 µl, 32 samples).
Absorbans and turbidity - end point, kinetics or spectral scanning.
Room temperatur to 45 °C, plate shaking.
DNA, RNA and protein analysis.
NanoDrop One, Thermo. UV-Vis.
Concentration and purity of DNA/RNA (1-2 µl).
NanoDrop
(Photo: Thermo Fisher Scientific)
Helos-alfa. For cuvettes, UV-Vis.
Telstar, LyoQuest with standard chamber.
Ice condenser capacity 5 kg/24 h.
Freeze drier
(Photo: Telstar)
Rotavapor and Speed Vacuum Concentrator
Buchi. Rotavapor R210 and Vacuum Controller V850.
Speed vacuum conentrator SC210A and Savant RVT 400 Refrigator Vapor Trap.
Precision balance
Mettler Toledo. Excellence Plus XP.
Range: 1µg – 6 g

Embedding system paraffin wax
Tissue processing machine – Tissue -Tek VIP
Embedding machine – Tissue-Tek TEC

Tissue-Tek TEC
(Photo: Sakura)

Tissue-Tek Tek
(Photo: Sakura)