The Imaging Centre - Mikroskopi laboratoriet
We cater for a wide range of life-science imaging requirements with expertise in animal cells, plants, microbes in materials science.
Kontaktinfo
E-post: imaging.centre@nmbu.no
Telefon: 67 23 27 61/62
Besøksadresse:
Oluf Thesens vei 43, 1433 Ås
Åpningstider:
Kl. 08:00 til 15.45
15. mai til 14. september:
kl. 08:00 til 15.00
About The Imaging Centre
The Imaging Centre, offers training and assignments related to Light Microscopy, Confocal Laser Scanning Microscopy, Laser Micro Dissection Microscope, Scanning and Transmission Electron Microscopy.
The Core Facility can provide guidance and technical expertise on the use of our entire infrastructure, to provide a low threshold for all users whether experienced or not.
The Centre is accessible to all internal and external users, both academic and industrial. We have various tissue preparation equipment available, and are currently updating our facilities to provide even better services in the future.
We cater for a wide range of life-science imaging requirements with expertise in animal cells, plants, microbes and limited experience in materials science.
For more images follow us on Instagram: @imaging.centre
Prices: We have different prices for use of the various instruments; please contact us by e-mail, and we will arrange for a meeting, or send you a pricelist.
Booking: Send us an e-mail, drop by the Imaging Centre or give us a call.
Contact information: imaging.centre@nmbu.no, phone: 67 23 27 61/62
Courses
Join us to learn more about the new confocal system at the Faculty of Veterinary Medicine. Open for everyone!
Tuesday 22nd of November
10.00-11.00 3D imaging with Leica Microscope –Thommie Karlsson
11.15-12.00 Confocal imaging with Stellaris –Simen Gylterud Owe
Location: event room “HKU2173” In VeterinærbygningenThe Imaging Centre are offering a two day course in confocal laser scanning microscopy.
Please send an e-mail to imaging.centre@nmbu.no if you are interested to join the next course, or have any questions.
Theory and hands-on training with both Leica CLSM SP5 and Zeiss LSM 800
The course give a thorough introduction to the use of light microscopy, confocal laser scanning microscopy, scanning- and transmission electron microscopy.
We have lectures, demonstrations and exercises on general and special optics, sample preparation, staining methods and all their various imaging techniques.
This course take place in the January block, and there will be given an oral exam in February/March.
Sign up by sending an email to: imaging.centre@nmbu.no
Admission has to be confirmed by email from this lab before the student can register for the course at the student office.
Here you can find more information about the BIO300 course. Please feel free to contact us if you have any questions.
The Imaging Centre are offering a two day course in light microscopy. Please send an e-mail to imaging.centre@nmbu.no if you are interested to join the next course, or have any questions.
Feel free to contact us if you need training on the microscope.
The course will give you a introduction to the diffrent techniques in light microscopy; bright field, dark field, phase contrast, differential interference contrast, polarization and fluorescent microscopy.
Equipment
The JEM-2100Plus is a multi purpose scanning transmission electron microscope, which combines the proven JEM-2100 optic system with an advanced control system for enhanced ease of operation.
The microscope is equiped with a LaB6-filament, TVIPS camera, an EDS system from Oxford, BEI, DF and BF detectors. High voltage range 80 - 200kV.
Axio Zoom.V16 combines a 16x zoom with a high numerical aperture of NA 0.25. It achieves a very high aperture in the medium zoom range, and you get superior brightness in large object fields.
With Axio Zoom.V16, the fluorescence zoom microscope for large samples you can view complete model organisms in fluorescence contrast.
The microscope is equiped with DAPI and GFP filtercubes.
The Zeiss LSM 800 confocal laser scanning microscope, has highly sensitive GaAsP detection, fast linear scanning and Airyscan detection.
Airyscan is the revolutionary detection concept from ZEISS to acquire perfect optical sections with a resolution of 120 nm (in x,y) and 350 nm (in z) even in thick samples.
With Airyscan's 4 – 8× better signal-to-noise ratio (SNR) you will be pushing sensitivity and superresolution imaging beyond the limits of all conventional confocals.
The microscope is upright, and has a chamber to control temperature if necessary.
Zeiss EVO 50 EP scanning electron microscope provides a flexible and productive tool for surface imaging at both high and low resolution.
Our SEM is a Extended Pressure (EP) instrument where the specimen environment can be controlled and we can examine fresh samples.
Detectors for Backscattered Electron Imaging (BEI) and X-ray analysis make it possible to detect and localize differences in element composition.
Spesifications:
Filament type: Tungsten (W)emitter
Resolution: 3.0 nm at 30 kV and 4.5 nm at 30 kV (BSD-EP mode)
Acceleration voltage: 0.2-30 kVWe have all the necessary equipment and expertise for sample preparation.
Responsible contact person is Hilde Kolstad.
The Leica TCS SP5 Confocal covers a broad range of requirements in confocal and multiphoton imaging. The system provides the full range (spot recording to real-time imaging) of scan speed at high resolution.
The TCS SP5 enables you to easily switch between conventional and resonant scanner.
With high-efficiency SP detection (five channels simultaneously) and optional AOBS(Acousto Optical Beam Splitter), the system delivers bright, noise-free images with minimal photo damage at high speed.
Specifications:
Available laser lines (nm):
Blue diode (BD): 405
Argon (Ar): 458, 476, 488, 496, 514DPSS: 561
Helium-Neon (HeNe): 633Available objectives:
Mag Numerical aperture Type Immersion medium 10x 0.3 HC PL FLUOTAR DRY 20x 0.7
HCX PL APO CSDRY 20x 0.7 HCX PL APO CS IMM 40x 0.85 HCX PL APO CS DRY 40x 1.25 HCX PL APO OIL 63x 1.4 HCX PL APO lambda blue OIL Inverted Leica DMI 6000 microscope
FRET and FRAP applications
Transmitted light detector - possibility to overlay transmitted light with multiple confocal images
There is no incubator (temperature- and gas control) available on the system
The Leica DM6B is a semi-automated research light microscope that provides all transmitted light contrast methods including automated differential interference contrast, polarization, phase contrast and fluorescense.
The microscope is equipped with a DMC4500 Digital Camera, and the new LAS X analysis program.
The filter systems available are D ET (UV/ violet), N2.1 (green) and GFP ET (green).
Leica LMD600 Laser Micro dissection microscope is a high-precision technology, for sample preparation to obtain well-defined starting material for downstream experiments in the fields of genomics, micro arrays, biochips, and proteomics.
Leica EM AFS 2 is an automatic freeze substitution system for embedding of samples following high pressure freezing and other cryo fixation methods, or progressive lowering of temperature (PLT) to substitute and resin infiltrate chemically fixed specimens.
The sample is polymerized under UV light and can be cut and immunelabeled for transmission electron microscopy. It is equipped with the Leica EM FSP automatic reagent handling system.
The Freeze substitution unit has an automatic reagent handling system, giving a one step preparation, reduced workload, and increased reproducibility.
It minimizes operators contact with toxic media and reduces the risk of losing specimens.
The Leica CM 1950 is a high quality motorized cryostat for sectioning at low temperatures. The sectionthickness and temperature can be precisely regulated, and adjusted to fit all kinds of samples.
The Leica VT1200 S is an automated vibratome. It has unique control and adjustments of unwanted vertical movements. You can section both fresh and fixed tissue.
Employees at the Imaging Centre
Hilde Raanaas Kolstad
General skills in the field of light microscopy; Light Microscope (LM), Confocal Laser Scanning Microscope (CLSM) and Laser Micro Dissection microscope (LMD).
Special skills for Transmission (TEM) and Scanning Electron Microscope (SEM).
Hilde has worked at the Imaging Centre since 2007, doing all kind of microscopy, preparationtechnices and teaching at the BIO 300 Microscopy Techniques.
Lene Cecilie Hermansen
Lene started working at the Imaging Centre in April 2015. She has broad experience from the former EM-lab at the Anatomy and Pathology Department at Adamstua, NMBU, and the The core facility for Advanced Electron Microscopy at OUH.
She has general skills in the different light microscopy techniques, preparation and staining methods, and specific skills in the field of transmission electron microscopy, preparation methods for pure morphological studies and for immunocytochemical localization of antigens on ultrathin sections.
Følg oss på instagram @imaging.centre