About the project

Aim

The main purpose of the project is to facilitate the development of new knowledge about connections and interplay between human and animal health. It will help to prevent disease and provide good health in both animals and humans. The term One Health expresses that there is a connection between human and animal health and the environment.

HUNT One Health is based on a parallel collection of stool samples from animals and participants in HUNT4 in the period 2017-2019. The samples are analyzed with modern sequencing technology so that the microorganisms in the samples and their properties can be revealed. Various research projects will have access to sequence data and other information from HUNT4 and HUNT One Health. In this way, we can better understand how the microbial communities of animals, humans and the environment interact. The parallel study of microbiomes in animals and animal owners at such a large-scale makes it possible to link the health status of animals and people and makes HUNT One Health a unique project.

Project group

The Project group is responsible for the management of the project with one representative from each institution:

  • Norwegian Veterinary Institute: Øivind Øines (project leader), Tel: 47611551,  Oivind.Oines@vetinst.no 
  • NTNU-HUNT4: Kristian Hveem: Tel: 74019242/ 47652530, Kristian.Hveem@ntnu.no 
  • NMBU Faculty of Veterinary Medicine: Ann-Katrin Llarena, Tel: 92089171,  ann-katrin.llarena@nmbu.no (deputy: Sabrina Rodriguez-Campos, Tel: 67231966, sabrina.rodriguez@nmbu.no)
     
    Former member: Eystein Skjerve (NMBU Faculty of Veterinary Medicine)

Steering group

        Former member: Merete Hofshagen (Norwegian Veterinary Institute)

Status and progress

The collection of sample material (including faecal samples) from animals and animal owners in HUNT4, was completed in the spring of 2019. After the sample collection, the project has continued to work on selecting suitable protocols for preparing the samples and arranging the research material for further use. About 3000 stool samples from dogs, cattle, sheep, horses and pigs were received from about 1700 pet owners. 550 animal owners have provided faecal samples from themselves and the animals. From production animals (cattle, pigs and sheep), approx. 1000 samples were received, and there are also many dog ​​samples and fewer from horses.
 

The samples were prepared for extraction of DNA from the microbes in the samples. A lot of work was needed to ensure that the information from the sample material can provide good data for research once the sequencing has been completed. One of the challenges was to find a good method to extract DNA from the samples stored in the freezer on filter paper. DNA from approx. 2500 samples was extracted and shipped to the company that has been granted the contract to perform the sequencing. Large amounts of data are generated when all the DNA from the microbes in the faeces is analyzed and we estimate that the project in this round will generate approx. 2.5 TB of data, with approx. 10 GB pr. sample. According to schedule all material will be sequenced by the end of August 2021 and will then be available to researchers.

Institutions and collaboration with HUNT4

The HUNT One Health project follows the same approach than HUNT. Material collected in the project will be made available to researchers and thereby contribute to the activity being expanded over time and HUNT One Health can become a player in the development of research within the One Health area. The project will invite researchers broadly, but hopefully there will be a core activity around the axis VI - NMBU - HUNT4/NTNU. 

Data access commitee (DAK)

The Data access committee is responsible for providing access to research data and material under HUNT One Health, in the same way as HUNT4. The DAK consists of the following members of the project group: Øines, Llarena/Rodriguez-Campos.

Participating researchers

Researchers from the Norwegian Veterinary Institute, NTNU/ HUNT and NMBU Faculty of Veterinary Medicine work closely together.

From the Veterinary Institute: Øivind Øines, Thomas Haverkamp, Camilla Sekse, Arne Holst-Jensen.

From NTNU/ HUNT: Kristian Hveem

From NMBU Faculty of Veterinary Medicine: Eystein Skjerve, Ann-Katrin Llarena, Yngvild Wasteson, Kristin Herstad, Ola Brynildsrud, Ane Mohn Bjelland, Ellen Skancke, Mari Røken, Anja Aardal, Sabrina Rodriguez-Campos, Silje Hansen, Anette Krogenæs, Anita Haug Haaland

Links


The Norwegian Veterinary Institute's webpage about the project (in Norwegian)

Article "To investigate connections between human and animal health" (in Norwegian)

 

 

 

 

Published 30. August 2021 - 13:06 - Updated 24. September 2021 - 16:18