Norwegian Research Council - HAVBRUK 2

Project number: 302543
Period covered - start date: 2020
Period covered - end date: 2024
Project's coordinator: NMBU   Volha Shapaval  


Project partners
NMBU: Faculty of Science and Technology (RealTek)
NMBU: Faculty of Chemistry, Biotechnology and Food Science (KBM)
NMBU: Faculty of Biosciences (BIOVIT)
California Center for Algae Biotechnology (UCSD), USA
Norilia AS, Norway
Borregaard ASA, Norway


Due to a rising global production of farmed fish, there is increasing demand for high-quality protein and energy sources for use in fish feed. In particular fish oil in aquaculture has increased dramatically in the last two decades. In industrially formulated feeds, aquaculture is already consuming more than 70% of globally produced fish oil, creating environmentally unsustainable demand that cannot be met by the limited pelagic fish catch. Due to stagnant production of fish oil from marine resources, the future needs for fish oil in aquaculture industry are expected to outstrip the current supply within the next decade.

Mature Atlantic salmon (left) and young fertilized salmon eggs (right)

Mature Atlantic salmon (left) and young fertilized salmon eggs (right)


Therefore, there is an urgent need for alternative and preferably land-based feed resourcess to replace the traditional fish oil sources. The OIL4FEED project aims at developing high-value oils for salmon feed from food by-products and lignocellulose biomass by use of oleaginous microorganisms. OIL4FEED will develop a process for producing microbial oils with a high level of ω-3 polyunsaturated fatty acids and ω-6 monounsaturated and polyunsaturated fatty acids by oleaginous microalgae and fungi from animal fat by-products and second generation sugars derived from Norwegian woody feedstock. This will allow, for the first time, to establish, a new value chain from "Norwegian by-products – to –Microbial Feed Oils" in Norway. This will increase financial and environmental sustainability of Norwegian aquaculture, agriculture, forest and food industry. OIL4FEED will deliver a complete set of new knowledge about the effect of algal/fungal oil on growth performance and health of fish.


Dzurendova S., Losada C.B., Dupuy-Galet B.X., Fjær K., Shapaval V.
Mucoromycota fungi as powerful cell factories for modern biorefinery
Applied Microbiology and Biotechnology (2021)

Gaykawad S.S., Ramanand S.S., Blomqvist J., Zimmermann B., Shapaval V., Kohler A., Oostindjer M., Boccadoro C.
Submerged Fermentation of Animal Fat By-Products by Oleaginous Filamentous Fungi for the Production of Unsaturated Single Cell Oil
Fermentation 7 (2021) 300

Dzurendova S., Shapaval V., Tafintseva V., Kohler A., Byrtusová D., Szotkowski M., Márová I., Zimmermann B.
Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy
International Journal of Molecular Sciences 22 (2021) 6710

Tafintseva V., Shapaval V., Blazhko U., Kohler A.
Correcting replicate variation in spectroscopic data by machine learning and model-based pre-processing
Chemometrics and Intelligent Laboratory Systems 215 (2021) 104350

Dzurendova S., Zimmermann B., Kohler A., Reitzel K., Nielsen U.G., Dupuy--Galet B.X., Leivers S., Horn S.J., Shapaval V.
Calcium affects polyphosphate and lipid accumulation in Mucoromycota fungi
Journal of Fungi 7 (2021) 300

Langseter A.M., Dzurendova S., Shapaval V., Kohler A., Ekeberg D., Zimmermann B.
Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi
Microbial Cell Factories 20 (2021) 59

Slaný O. , Klempová T., Shapaval V., Zimmermann B., Kohler A., Čertík M.
Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation
Microorganisms 9 (2021) 170

Dzurendova S., Zimmermann B., Tafintseva V., Kohler A., Horn S.J., Shapaval V.
Metal and Phosphate Ions Show Remarkable Influence on the Biomass Production and Lipid Accumulation in Oleaginous Mucor circinelloides
Journal of Fungi 6 (2020) 260

Slaný O., Klempová T., Shapaval V., Zimmermann B., Kohler A., Čertík M.
Biotransformation of Animal Fat-By Products into ARA-Enriched Fermented Bioproducts by Solid-State Fermentation of Mortierella alpina 
Journal of Fungi 6 (2020) 236

Published 9. April 2020 - 12:42 - Updated 17. December 2021 - 16:35