KJB420 Proteomics II

Credits (ECTS):10

Course responsible:Morten Skaugen

Campus / Online:Taught campus Ås

Teaching language:Engelsk, norsk

Limits of class size:12 (KJB320 + KJB420)

Course frequency:The course will be given in uneven years only.

Nominal workload:Lectures 16 hrs. Laboratory work 22 hrs. Presentation 6 hrs. Thesis 40 hrs. Self study 216 hrs.

Teaching and exam period:This course starts in the January block. This course has teaching in the January block + teaching/evaluation in the spring parallel.

About this course

Sample preparation for MS- and LC-MS analysis of proteins, identification and quantification of proteins. It is emphasized that this course requires the ability and willingness to work independently and meticulously with advanced biochemical methods.

The practical part of the course introduces modern methods for for sample preparation, data acquisition and data analysis. The biological material used in the lab is relevant for current issues in medicine as well as bioremediation and energy production (UN sustainable development goals 3, 7, and 12).

Students will present one scientific article in a seminar. If necessary, this presentation must be given in English.

Learning outcome

Knowledge:

The student should have acquired a good understanding of the concepts of proteomics as an integrated part of an -omics approach to biological/biochemical analysis, and as distinct from classical protein analysis. The students should also have acquired a good understanding of the significance of sample preparation and basic modern mass spectrometric analysis of protein and peptide samples. In addition, the students should know the principals of bioinformatic data analysis and interpretation of proteomics data.

Skills:

The students should have acquired fundamental skills in the laboratory, both in terms of sample preparation and mass spectrometric analysis of samples from different cell types/organisms. They should also be able to use a selection of data analysis tools to convert raw data to useful biological/bichemical information. The student is able to perform the preparations, techniques and analyses employed in the course. The student masters the instruments and equipment used in the course.

General Competence:

The students should have acquired a fundamental understanding of the proteomics discipline, to a point where they should be able to employ this analysis platform in their own work.

  • Lectures. Laboratory exercises at a high level of independency, with journal. Writing of a small thesis. Oral presentation of a topic or an article.
  • Contact with teachers during lectures and exercises.

    E-mail: morten.skaugen@nmbu.no

    Canvas.

    • Biochemistry equivalent to KJB201.
    • Completed master degree in chemistry, biotechnology or equivalent qualifications
  • Portfolio assessment consisting of laboratory report, oral presentation and data analysis assignment.
    • An external examiner evaluates the course arrangements and laboratory reports
    • Internal examiners evaluates the oral presentations and data analysis assignment.
  • Participation in all activities (January block and spring parallel). Approved lab report, data analysis report and presentation of topic or article.
  • Participation in all classes is mandatory.

    The course is given in uneven years only.

  • January block: Intensive course for two weeks, including 16 hrs of lectures, 22 hrs of laboratory work and an oral presentation (6 hrs).

    Spring parallel: Special exercise with report/thesis: 60 hrs.

  • The course is reserved for PhD-students.
  • KJB420 is an extended version of KJB320. Students with KJB320 get 5 credits for KJB420.
  • Passed / Not Passed
  • Special requirements in Science