Course code KJB201

KJB201 Laboratory Course in Biochemistry

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Showing course contents for the educational year starting in 2019 .

Course responsible: Vincentius Gerardus Henricus Eijsink
Teachers: Heidi Østby, Tora Asledottir, Bjørge Westereng, Eirik Garpestad Kommedal, Ellen Cathrine Hasle Kokkim, Anne Cathrine Bunæs, Tove Gulbrandsen Devold, Silje Benedicte Lorentzen, Marianne Slang Jensen, Irene Comi, Eirik Garpestad Kommedal
ECTS credits: 5
Faculty: Faculty of Chemistry, Biotechnology and Food Science
Teaching language: NO
(NO=norsk, EN=Engelsk)
Limits of class size:
108 (including students on KJB210)
Teaching exam periods:
This course starts in the Spring parallel. This course has teaching/evaluation in the Spring parallel, .
Course frequency: Annually
First time: 2005V
Preferential right:
Students that have KJB201 as an compulsory subject will have prioritry when allocating labspace.
Course contents:

The laboratory course is built up as follows: 8 exercises, each lasting about 6 hours, one exercise per week.

Lectures (14 hours).

Writing of six journals, length 8 - 15 pages. Simple laboratory reports (fill-in form) for the other excercises.

Two or three students per group.

Learning outcome:

KNOWLEDGE:

Students will learn about elemental biochemical techniques such as electrophoresis and chromatography and get a theoretical introduction to the principles on which these techniques are based. In addition, the course covers a few areas of applied biochemistry, especially enzyme technology. The course also provides some specialization in parts of KJB200, including enzyme kinetics.

SKILLS:

Students will learn how to perform a variety of laboratory techniques, including:

Pipetting small volumes

Elemental spectrophotometry

Standard curves

Measurement of protein concentration

Measurement of enzyme activity, including enzyme kinetics

Chromatography (purification) of proteins

Gel electrophoresis of proteins

Measurement of antioxidants and vitamin C

Studies of viscosity and gels.

Apart from technical skills, one learns to critically evaluate methods and own research results.

Journal writing is an important part of the course and after completing the course one should be able to report research results in a scientific manner.

GENERAL COMPETENCE:

General laboratory skills - How to work in a laboratory.

Critical evaluation and professional reporting of methods and own research results.

Journal writing.

Because the work is done in groups the course gives training in doing research in teams.

Learning activities:
Lectures. Exercises in laboratories (with intensive guidance). Writing of journals.
Teaching support:

Question hours each week. Intensive guidance for journal writing.

Canvas.

Syllabus:

Compendium written by teachers and revised each year.

Lecture notes.

Prerequisites:
General chemistry equivalent to KJM100. Biochemistry equivalent to KJB200 (can be taken the same semester).
Recommended prerequisites:
Organic chemistry equivalent to KJM110.
Mandatory activity:
All lectures about the laboratory course (14 hours).Participation in the laboratory exercises.Handing in  the laboratory journals.
Assessment:

Laboratory journals count 25 % and a written test (2 hrs) counts 75 %.

Both parts must be passed.

Nominal workload:

Lectures: 14 hours.

Laboratory exercises, with preparation and writing of laboratory journals: 96 hours.

Individual study: 40 hours.

Entrance requirements:
Special requirements in Science
Reduction of credits:
It is not possible to receive credits for both KJB201 and KJB210. KJB210 incorporates KJB201.
Type of course:

Lectures: 14 hours.

Laboratory work: 6 hours per week for 8 weeks.

Examiner:
An external examiner approves the examination questions and marks a minimum of 25 selected examinantion papers. The internal examiner grades the laboratory journals.
Allowed examination aids: B1 Calculator handed out, no other aids
Examination details: Continuous exam: A - E / Ikke bestått