Course code BIO332

BIO332 Experimental Molecular Microbiology

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Showing course contents for the educational year 2018 - 2019 .

Course responsible: Åsa Helena Frostegård
Teachers: Morten Kjos, Daniel Straume, Inga Leena Angell, Anja Ruud Winther, Pawel Michal Lycus, Ine Storaker Myrbråten, Gro Anita Stamsås, Linda Helén Godager, Linda Liberg Bergaust, Else Marie Aasen, Leiv Sigve Håvarstein, Zhian Salehian, Rannei Tjåland, Knut Rudi
ECTS credits: 10
Faculty: Faculty of Chemistry, Biotechnology and Food Science
Teaching language: EN, NO
(NO=norsk, EN=Engelsk)
Limits of class size:
Teaching exam periods:
This course starts in January block. This course has teaching/evaluation in January block + teaching/evaluation in Spring parallel.
Course frequency: Annually
First time: Study year 2009-2010
Preferential right:
The course is compulsory for students who are admitted to the master´s programme in Biotechnology, specialisation microbiology. 
Course contents:

This is an intensive laboratory course over three weeks in January. A few lectures/seminars will be given in the January block, the rest will be given during the spring term. A total of 18 lectures will be given. In addition, a number of scientific primary articles relevant for the experimental work will be discussed in seminars during the first part of the spring semester. The students write a report from the laboratory exercises, in which relevant scientific literature is referred. The laboratory exercises cover methods used for:

  • measurement of gene expression and biological diversity in various ecosystems
  • characterisation and identification of bacteria
  • horizontal gene transfer
  • genetic regulation
  • genome sequencing
  • interpretation of results and discussions on the various methods

Students write an individual, final report.

Learning outcome:
The students will become familiar with techniques used in microbiological research, with emphasis on molecular methods. Students will become aware of the contexts in which the different methods are used, and the strong and weak points of the various methods will be discussed. In addition, they will learn how to interpret and evaluate biological data, and gain experience in reading and using scientific primary literature. The students will learn and obtain experience with how to prepare and deliver effective oral and written presentations of technical information and scientific results. They will learn to think critically and solve complex and multidisciplinary problems, as well as learn to accurately interpret current research literature.
Learning activities:

Laboratory exercises. Seminars. 

The seminars are based on articles describing relevant scientific findings or methods. The students actively participate in discussions during these seminars. The main focus of the course is on learning methods used to elucidate modern microbiological questions. Special lectures are given in connection with the laboratory exercises.

Teaching support:
The teachers are present during the entire course period and can also be reached via e-mail. Canvas.
Selected scientific articles will be discussed during the seminar series. Scientific articles are used in an extensive report from the laboratory exercises and are also curriculum for the oral examination.
Biochemistry equivalent to KJB200. General microbiology equivalent to BIO230. Molecular biology equivalent to BIO210.
Recommended prerequisites:
The laboratory courses BIO233 and BIO211 (or equivalent).
Mandatory activity:
Lectures, seminars and laboratory exercises.
Written exam where the curriculum will be the topics from the lab course and selected scientific papers on relevant topics. External examiner.
Nominal workload:
Laboratory work: 120 hrs. Seminars/lectures: 18 hrs. Self study and writing of laboratory repport: 162 hrs.
Entrance requirements:
Special requirements in Science
Type of course:

January block: three weeks (120 hrs) laboratory course, with some lectures.

Spring parallel: 2 seminars /lectures per week (2x2h) in the first half of the parallel, will be announced on Canvas.

Submission of written report: week 9. Oral exam: week 13, 14 or 15, depending on when it is Easter week.

Students who have not taken laboratory courses in microbiology before at NMBU have to pass a small test on laboratory safety to participate in the course. The test will be given in December.

External examiner of the report on independent assignment.

External examiner evaluate the oral exam.

Allowed examination aids: A1 No calculator, no other aids
Examination details: Continuous exam: A - E / Ikke bestått