BIO300 Microscopy Techniques
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Showing course contents for the educational year starting in 2016 .
Course responsible: Yeon Kyeong Lee
Teachers: Hilde Raanaas Kolstad, Hilde Raanaas Kolstad, Lene Cecilie Hermansen
ECTS credits: 10
Faculty: Faculty of Biosciences
Teaching language: EN, NO
Limits of class size:
Minimum 6 students. Maximum 15.
NB! Sign up by sending an e-mail to: firstname.lastname@example.org. Admission must be confirmed by the Imaging Centre. The Imaging centre is responsible for the registration at the Student office.
Teaching exam periods:
The course starts in the January block. Teaching and assessment in January block, and exams in February/March spring parallel.
Course frequency: <p>Annually</p><p>Sign up by sending an email to: email@example.com.</p>
First time: 2004V
Students at higher levels requiring microscopy techniques in their assignment.
¿ General optics in: electromagnetic radiation, resolving power, magnification, refractive index, diffraction, phase shift, interference.
¿ Special optics: general structure of the microscope and ray paths in LM (light microscopy), CLSM (confocal laser scanning microscopy), SEM (scanning electron microscopy) and TEM (transmission electron microscopy).
¿ Different optical and contrastenhancement methods in LM, CLSM, SEM and TEM.
¿ General preparationmethods in LM, SEM, CLSM and TEM
¿ Lectures, demonstrations and exercises gives a thorough introduction in the use of microscopes and their various imaging techniques.
Students will have acquired theoretical and practical knowledge in optics, sample preparation and image formation so that they can:
1) explain the central optical terms (resolving power, magnification and more)
2) describe the beam path and imaging in various microscope types
3) make rational choices in sample preparation and imaging methods based on sample type and problem
4) evaluate and interpret different micrographs (own or published).
Students will be able to diagnose, adjust and use the following microscope:
1) Light microscope, with various types of optics (brightfield, darkfield, polarization, phase contrast, interference contrast and fluorescence)
2) Confocal laser scanning microscope
3) Scanning electron microscope, in various imaging mode (SEI and BEI) and X-ray analysis.
4) Transmission electron microscope
Students will also be able to use the following equipment for sample preparation:
1) Ultramicrotome: sectioning for LM and TEM
2) Cryostat: sectioning for LM, SEM and laser micro-dissection
3) Critical point drying and sputter coating for SEM
Students will also be able to use a variety of methods in sample preparation, dyeing, labeling and basic imaging.
Lectures, online self-study, practical training, demonstrations, and laboratory exercises.
Lectures, compendium and selected Web-pages.
Bachelor in the field of natural sciences.
Bachelor in the field of natural sciences
Exercises and demonstrations.
Submission of written journal counts for 1/4 of the final grade. Oral examination counts for 3/4 of the final grade. The journal must be approved before the student can take the exam.
Type of course:
Lectures: 10-12 hours per week. Demonstrations: 2-3 hours per week. Training: 10-12 hours per week Exercises: 10-15 hours per week. Additional lectures: Internet-based individual study and journals. Practical work ends with a written exercise/journal.
Examination details: Term Paper: A - E / Ikke bestått