BIO244 Plant Biotechnology: Cell- and Tissue Culture and Genetic Modifications
Showing course contents for the educational year 2019 - 2020 .
Course responsible: Trine Hvoslef-Eide
Teachers: Linda Ripel, Gry Skjeseth, Astrid Helene Sivertsen, Tone Ingeborg Melby
ECTS credits: 5
Faculty: Faculty of Biosciences
Teaching language: EN, NO
Limits of class size:
The course has laboratory exercises and for this reason we have to limit the student participation to 18 each year. If you are unable to attend one year, we can put your name on the waiting list for next year. Students who are enrolled in the program Plant Sciences have the priority to attend because this is an obligatory course for these students.
Teaching exam periods:
This course starts in Spring parallel. This course has teaching/evaluation in Spring parallel .
Course frequency: Annually
First time: Study year 2013-2014
Plant Sciences, Bachelor and Master, subsequently Biology and Biotechnology students, Bachelor and Master
The course is offered through lectures and laboratory exercises. The students will have somewhat uneven hours in the laboratory exercises depending on the theme of the week. The course is coordinated with BIO245 and BIO246 regarding the dates for hand-ins.
The lab exercises will be in smaller student groups. We expect the students to have read the chapter in the lab exercise book on the goal and methods prior to the lab. The students follow their own cultures through the semester and can take their own plants home at the end (except GM plants). Lectures and practicals within: Celle- and tissue culture: In vitro propagation through adventitious and axillary propagation (different tissues), disease elimination through meristem cultures, chimeras and their importance in propagation, somatic embryogenesis, genetic modifications. , markers and gene expression (gene silencing). Lectures within the following themes: Disease elimination through meristem cultures, chimeras and their importance in propagation and breeding. Genetically modified crops: Current and potential role in plant production, potential and limitations of different types of transgenes, laws and regulations in Norway (and EU), internationally related to genetically modified (GM)crops. Gene editing (CRISPR). Use of markers and gene expression (gene silencing).
There is a possibility for the students to expand the course with a thematic essay on a chosen topic (an extra 5 ects for BIO246). In BIO246, the students can choose to write ohn a topic from either BIO244 or BIO245.
The course will introduce the students to the different biotechnological methods used in plants and their role in modern research in biology as well as in the production of food, feed and other plant products. The students should be able to understand how biotechnology may supplement or replace traditional methods in propagation and breeding. The course consists of both lectures and laboratory exercises, the themes covered are: Celle- and tissue culture: In vitro propagation through adventitious and axillary propagation (different tissues), chimeras and their importance in propagation, somatic embryogenesis, double haploids, gene modifications, gene expression. Through lectures only: disease elimination through meristem cultures,Genetically modified crops: Current and potential role in plant production, potential and limitations of different types of transgenes, laws and regulations in Norway (and EU) related to genetically modified (GM)crops and gene editing (CRISPR).
There will be lectures, laboratory exercises. This will be presented in the form of a written report which will be evaluated by an external examinor and the main teacher.
We actively use Canvas - the new learning platform at NMBU for support, all lectures are placed there (in Norwegian and English). The practical teachers are available between classes in the laboratory and the main teacher is available through e-mail and by appointment.
The lectures are video taped and are available for the students to revise when writing their lab reports
Slater, A., Scott, N. and Fowler, M. (2008). Plant Biotechnology, The Genetic Manipulation of Plants, Oxford University Press Inc., 2nd Edition. PLUS hand-outs in class. Canvas will tell you what has been handed out for your semester.
Basic knowledge of genetics (BIO120) and plant physiology (BOT130). It is the student's responsibility to have the necessary knowledge beforehand, or study alongside on their own.
The practicals in the lab are obligatory and neccessary to gain full benefit from this course. The lab reports are the basis for the evaluation and hence obligatory too, even for those which you may have had a valid excuse for not attending in person.
Written lab report to be written according to the set up for a scientific publication within our field. No formal exam for this course.
The semester consists of 11-12 double lectures of 2 x 45 min each, pluss 25-30 hours exercises in the lab. The students will contribute approx 100 hours in the form of preparing for classes (esp lab classes) and writing up the lab reports.Total 150 hours work.
Basic knowledge in Natural Sciences
Reduction of credits:
BIO243 (5 credits)
Type of course:
There will be a double lecture and 3 hours laboratory course every week, on average.
This course is part of a package of three 5 ects courses, BIO244, BIO245 and BIO246, but you may take only one or two of them.
We use the external examiner actively to secure a good structure and the evaluation of the hand-in report from the practicals
Examination details: Continuous exam: A - E / F