DYR214 Laboratory Diagnostics
Credits (ECTS):5
Course responsible:Hege Cathrine Mittet Brun-Hansen
Teaching language:Norsk
Course frequency:Annually
Teaching and exam period:See semester schedule
About this course
To prepare the student on the laboratory tasks an animal nurse will meet in the clinic. The subject will give an understanding of both practical and theoretical aspects associated with sampling and further treatment of the different types of test material for diagnostic and shipment
Learning outcome
The student should:
- Be able to use light microscope and know how a "Köhler" setting is performed.
- Be able to perform pipetting and know how a pipette is adjusted.
- Be able to calibrate a refractometer.
- Know how blood samples must be treated for diagnostics, storage and shipment, and be able to safeguard the test materials in a way that is gentle on the materials and gives the greatest probability for the most correct test results.
- Be able to assess if a blood sample is of diagnostic quality, and to perform differential count in the blood smear.
- Be able to identify platelet clumps and estimate the platelet count in the blood smear.
- Be able to perform bacteriological analysis using Uricult, including analysing the result.
- Be able to perform streak plate techniques on solid growth media according to aseptic guidelines.
- Be able to Gram stain bacterial cultures and differentiate between Gram-positive and Gram-negative cells as well as determine cell morphology by use of light microscopy.
- Know the basic principle of antimicrobial susceptibility testing and interpretation.
- Have knowledge on relevant factors that can affect the reliability of a urine test.
- Be able to prepare urine samples for diagnostic and shipment, and have knowledge about the most common diagnostic findings on microscopic examination of sediment.
- Be able to perform urine stix analysis and specific weight by refractometry.
- Know about the principles and procedures for taking and preparation of skin samples and be able to identify the most common parasites and diseases in and on the skin.
- Be able to take stool samples or instruct owner to do this for virological, bacteriological and parasitological examination.
- Be able to perform flotation, McMaster and SNAP test techniques on fecal samples to detect parasites.
- Know about the principles around treatment and shipment of different types of biopsies, including fine needle aspiration.
- Be able to correctly treat a semen sample and have knowledge on how sampling of semen is performed. The student must also know how a semen sample is evaluated.
- Know about the principles and methods for quantification of immunoglobulins in serum or plasma.
- Have knowledge about principles behind and execution of rapid tests for measuring inflammation and autoimmunity in dogs.
- The student should know how to maintain and treat diagnostic equipment, including microscopes, centrifuges and refractometer.
- Know the main principles for blood transfusion and how to test recipient and donor before transfusion.
The course is a 2 week theoretical and practical teaching followed by one week where there will be arranged a practical exam.
The teaching will be a combination of theory and practice where the students learn how different samples are taken and how they should be treated to give reliable results.
- Clinical practice including simple laboratory course from the first year of study. External practice and the subject DYR206: Pathology and animal nursing
Practical exam.
Pass/fail
- Mandatory participation to course activity and lectures.
Requirements for eqipment:
Practical teaching is given in the laboratory auditorium. Lab coats are mandatory.
- B-DYR