BIO211 Laboratory Course in Molecular Biology
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Showing course contents for the educational year 2020 - 2021 .
Course responsible: Bao Dung Diep
Teachers: Ingvild Reinseth, Gro Anita Hvidsten Stamsås, Siri Merete Grønhovd, Tina Rise Tuveng, Lars Fredrik Moen
ECTS credits: 5
Faculty: Faculty of Chemistry, Biotechnology and Food Science
Teaching language: NO
Limits of class size:
Maximum 48 students, prioritized as follows:students who have passed the course BIO210 (or equivalent)students who have passed the course BIO210 (or equivalent) and have BIO211 as a mandatory course in their education planthe grade obtained on the course BIO210 (or equivalent course)
Teaching exam periods:
This course starts in June block. This course has teaching/evaluation in June block.
Course frequency: Annually
First time: Study year 2005-2006
- the Bachelor's program in Biotechnology
- the Master's program in Technology, specialisation Chemistry and Biotechnology
- the Master's program in Food Science, specialisation Food and Health
The course consists of 3 independent laboratory assignments. Techniques used
- Antimicrobial assays
- DNA cloning
- Protein purification
- DNA and protein sequence analyses
The students write extensive laboratory reports after each assignment, covering
- description of the experiments
- presentation of the results
- discussion of the results (both positive and negative results)
Students will gain insight on and hands-on experience with some central technologies in molecular biology, such as PCR, cloning, gel electrophoresis, bioinformatic tools for sequence analyses, and purification of DNA and protein. Another central aspect of the course is to learn how to write scientific reports based on experimental data.
Students can make use of their theoretical knowledge and lab-experience to solve some simple biological problems, such as to use bioinformatic tools to search for sequences, to do sequence analysis, to design and clone a gene, to control gene expression, to measure biological activity, and to follow and perform protocols for DNA and protein purification and analysis.
Acquired skills can later contribute in the development of various molecular tools to solve important problems in the society or in the environment. E.g., to
- improve health by producing vitamines and better and healthier food,
- secure life quality on land and in waters by removing toxic pollutants through enzymatic processes,
- produce more food using genetic technologies, and
- develop new medicines for human and veterinary use.
These opportunities and challenges are central in many UN sustainable development goals.
Students should (i) have an overview of and the ability to use central molecular tools in bioresearch, (ii) have increased self-confidence in labwork, (iii) be able to work in research, on projects with supervision, and (iv) be able to write reports based on experimental data.
Laboratory course.Short lectures will be given before each laboratory exercise and the results will be discussed.Journal writing.Practical guidance and assistance during the course is given by experienced teachers.
4-6 teaching assistants will be present for academic guidance during the course. Canvas.
Compendium: "Exercises in molecular biology BIO211". The NMBU Bookstore.
Students must have passed the course BIO210 Molecular Biology, or have approved knowledge equivalent to BIO210.
- Cell biology equivalent to BIO100.
- Genetics equivalent to BIO120.
- Microbiology equivalent to BIO130.
Compulsory laboratory exercises. Students must be present at all time during the course. Otherwise the course will not be approved.
Written laboratory journals; all must be approved.
Lectures and reviews: 20 hours. Laboratory exercises and preparation: 90 hours. Journal writing: 40 hours.
Special requirements in Science
Type of course:
Two weeks' intensive course: Laboratory assignments will be discussed before they are given. Laboratory work will be explained and followed up on. The class will review and discuss laboratory results. Writing of journals during the course.
Unless otherwise notified, the lab part of the course will take place during the first two weeks of June-block. Deadline to submit the reports is two weeks after the lab part.
Students must be present all day during the course. It is not possible to include other activities during the course period.
The written exam in BIO210 (or an equivalent course) must be passed before the student can enter the course. This arrangement exists for two reasons: to ensure that the student's theoretical knowledge is sufficient to conduct practical exercises, and to maintain the proper microbial security of the course.
Maximum 48 students, prioritized as follows:
- students who have passed the course BIO210 (or equivalent)
- students who have passed the course BIO210 (or equivalent) and have BIO211 as a mandatory course in their education plan
- the grade obtained on the course BIO210 (or equivalent course)
An external examiner approves the laboratory exercises. Internal examiners grades the laboratory journals.
Examination details: :