Ionizing radiation exposure of parents induces harmful effects in their unexposed progeny. The effects include developmental disturbances, reproduction impairments and neurological disorders that may persist for several generations.
This PhD project will investigate the molecular toxic effect mechanisms in the C. elegans model organism with emphasis on three connected topics:
- Effects on epigenetic programs related to defects in spermatogenesis.
- DNA repair and DNA damage tolerance mechanisms conferring resistance to genotoxic stress in C. elegans embryos.
- The influence of radiation exposure on development of ageing phenotypes and models of age related disease (Parkinson disease) in irradiated nematodes and their offspring
The main tasks of the PhD will be:
- To plan and execute irradiation experiments using C. elegans
- Quantify genotoxic, reprotoxic and neurological effects
- Perform microscopy analysis of molecular and phenotypic effects
- Analyze expression of key epigenetic regulatory genes
- Analyze RNAseq data
The experimental work will take place at both NMBU and EpiGen.
The project is a collaboration between CERAD Centre of Excellence for Environmental Radioactivity (MINA/NMBU & Norwegian Institute of Public Health/NIPH) and The Institute of Clinical Medicine (EpiGen), Department of Clinical Molecular Biology, UIO, Akershus University Hospital.