After a nice collaboration with colleagues in Japan and China, we establish a tool for gonadectomy and blood sampling in the medaka. This tool can help answers key questions regarding roles of sex steroids in the control of brain-pituitary-gonad axis. The article describes the step-by-step procedures of how to gonadectomize male and female medaka as well as to sample the blood repeatedly.
Full article plus video: Gonadectomy and blood sampling procedures in the small size teleost model Japanese medaka (Oryzias latipes). J. Vis. Exp. 166, e62006. doi 10.3791/62006