KJB430 Protein redox chemistry
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Showing course contents for the educational year starting in 2020 .
Course responsible: Åsmund Røhr Kjendseth
ECTS credits: 4
Faculty: Faculty of Chemistry, Biotechnology and Food Science
Teaching language: EN, NO
Limits of class size:
Teaching exam periods:
May - June 2021
Course frequency: Even years (the 2020 course will be moved to May-June 2021 because of the SARS-Cov2 outbreak).
First time: 2017H
PhD students registered with BioCat (https://site.uit.no/biocat/)
This course aims to provide students with both theoretical knowledge and practical skills in biological redox chemistry. The plethora of different redox cofactors that assist complex chemistry in enzyme active sites include transition metal ions, organic molecules and amino acid side chains. Moreover, the protein scaffold modulates the functionality of these cofactors, for example are iron ions and flavin involved in both electron transfer processes and catalysis. Redox active enzymes have a large potential to increase the sustainability of industrial processes by reducing pollution, improving waste treatment and facilitating the use of bioenergy. The students will be trained in basic electrochemistry, enabling them to recognize and identify "standard" challenges that tend to appear when working with red-ox enzymes. The students should after completing the course be able to carry out initial red-ox enzyme characterizations independently and be familiar with further experimental opportunities.
After completing the course the students should understand basic electrochemical theory and be familiar with protein bound cofactors that are involved in redox reactions. The students should also be able to experimentally determine what redox cofactor(s) that is present in a protein sample. Finally, the students should be able to design an experimental approach for characterizing a redox active protein with methods as UV-vis spectroscopy, voltammetry and stopped-flow.
Lectures, theoretical exercises and practical exercises.
Teachers will be available every day through the course period. Canvas - Learning Management System.
Provided material. This will include articels and manuals.
Master's degree including courses in:
- General chemistry equivalent to KJM100
- General biochemistry equivalent to KJB200 and KJB201
- Experience from practical lab-work
Course in physical biochemistry corresponding to KJM230.
Lectures, theoretical exercises, practical exercises and a final report.
Evaluation of final report (pass/failed)
A total of 120 hours:
- Lectures: 10 h
- Theoretical exercises: 12 h
- Prectical exercises: 18 h
- Self study: 80 h
The lectures and practical exercises are all given in one week.
PhD students in natural sciences
Type of course:
One week including
- 10 hours lectures
- 12 hours theoretical exercises
- 18 hours practical exercises
This is a one-week course in May / June 2021
Internal examiners revise the final report and grade the exam (pass/failed).
An external examiner approves the course's content and form of evaluation.
Examination details: Continuous exam: Passed / Failed