Course code KJB430

KJB430 Protein redox chemistry

Check for course changes due to the coronavirus outbreak on Canvas and StudentWeb.

Norsk emneinformasjon

Search for other courses here

Showing course contents for the educational year starting in 2017 .

Course responsible: Åsmund Røhr Kjendseth
Teachers: Bastien Pierre André Bissaro, Ingvild Isaksen, Morten Sørlie
ECTS credits: 4
Faculty: Faculty of Chemistry, Biotechnology and Food Science
Teaching language: EN
(NO=norsk, EN=Engelsk)
Limits of class size:
10
Teaching exam periods:
May 28 - June 1, 2018
Course frequency: Even years.
First time: 2017H
Preferential right:
PhD students registered with BioCat (https://site.uit.no/biocat/) 
Course contents:
This course aims to provide students with both theoretical knowledge and practical skills in biological redox chemistry. The plethora of different redox cofactors that assist complex chemistry in enzyme active sites include transition metal ions, organic molecules and amino acid side chains. Moreover, the protein scaffold modulates the functionality of these cofactors, for example are iron ions and flavin involved in both electron transfer processes and catalysis. The students will be trained in basic electrochemistry, enabling them to recognize and identify "standard" challenges that tend to appear when working with red-ox enzymes. The students should after completing the course be able to carry out initial red-ox enzyme characterizations independently and be familiar with further experimental opportunities.
Learning outcome:
After completing the course the students should understand basic electrochemical theory and be familiar with protein bound cofactors that are involved in redox reactions. The students should also be able to experimentally determine what redox cofactor(s) that is present in a protein sample. Finally, the students should be able to design an experimental approach for characterizing a redox active protein with methods as UV-vis spectroscopy, voltammetry and stopped-flow.
Learning activities:
Lectures, theoretical exercises and practical exercises.  
Teaching support:
Teachers will be available every day through the course period. 
Syllabus:
Provided material. This will include articels and manuals.
Prerequisites:

Master's degree including courses in:

- General chemistry

- General biochemistry

- Experience from practical lab-work

Recommended prerequisites:
Course in physical biochemistry
Mandatory activity:
Lectures, theoretical exercises, practical exercises and a final report.
Assessment:
Evaluation of final report and an oral exam. (pass/failed)
Nominal workload:
In total 120 hours. The lectures and practical exercises are all given in one week. 
Entrance requirements:
PhD students in natural sciences
Reduction of credits:
-
Type of course:

One week including

  • 10  hours lectures
  • 12 hours theoretical exercises
  • 18 hours practical exercises 
Examiner:

Internal examiners revise the final report and grade the exam (pass/failed).

An external examiner approves the course's content and form of evaluation. 

Examination details: Continuous exam: Bestått / Ikke bestått